DEMIANCHUK OLEH
PhD. student, assistant of Department of Biochemistry and Biotechnology
Vasyl Stefanyk Precarpathian National University
ADDRESS
Address: Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ministry of Education and Science of Ukraine, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
e-mail: oleh.demyanchuk@gmail.com
Tel./Fax: +38(0342)596171.
Scopus: https://www.scopus.com/authid/detail.uri?authorId=57217488002
ORCID: https://orcid.org/0000-0002-4730-0807
Google Scholar: https://scholar.google.com.ua/citations?user=FQJOg6sAAAAJ&hl=uk
EDUCATION
Master’s thesis: Optimization of conditions for the determination of proteins by the method of semi-dry Western blot, September 2019 – December 2020, Department of Biochemistry and Biotechnology, Faculty of Natural Sciences, Vasily Stefanik Precarpathian National University.
Scientific adviser: doctor of scientific sciences, professor Bailyak MM
Thesis: Participation of the redox system Nrf2 / Keap1 in the regulation of development and resistance of fruit flies to toxicants, September 2018 – June 2019, Department of Biochemistry and Biotechnology, Faculty of Natural Sciences, Vasily Stefanik Precarpathian National University.
Scientific adviser: doctor of scientific sciences, professor Bailyak MM
Full course of study in Biology (Bachelor’s degree) of the Faculty of Natural Sciences, Precarpathian Vasily Stefanik National University, 2015–2019.
Full course of study in Biology (Biochemistry) (Master degree) of the Faculty of Natural Sciences, Vasily Stefanik Precarpathian National University, 2019–2020.
CAREER
2020-2024 – Leading Specialist of the Department of Biochemistry and Biotechnology, Faculty of Natural Sciences, Vasyl Stefanyk Precarpathian National University
2023- present – Assistant professor of the Department of Biochemistry and Biotechnology, Faculty of Natural Sciences, Vasyl Stefanyk Precarpathian National University
SCIENTIFIC INTERESTS
– oxidative stress and antioxidant protection in living organisms
– mechanisms of adaptation to various stresses, in particular to oxidative stress in the living
organisms
– aging
CURRENT RESEARCH WORK
– changes in redox homeostasis in the body of living organisms
– biochemical mechanisms of adaptation of organisms to stressful conditions
– study of the effect of alpha-ketoglutarate and plant extracts on aging
– study of the effect of high-calorie diets on biochemical and physiological parameters in the body of mice and changes in the behavior of mice.
LABORATORY METHODS
– Derivation and maintenance of laboratory lines D. melanogaster; determination of life expectancy of fruit flies, determination of physiological indicators related to age (motor activity, sensitivity to stressors, fertility, etc.)
– Conducting experiments with behavioural tests on the mice (open field test, elevated plus maze (EPM), etc.)
– Preparation of extracts and homogenates of tissues, determination of enzyme activity, determination of concentration of glycolysis metabolites, tricarboxylic acid cycle, etc.; protein electrophoresis; western blotting; spectrophotometric determination of the main indicators of oxidative stress.
AWARDS
2018 – 3rd place for the best poster presentation at the XIV International Conference of Students and Postgraduates “Youth and Progress in Biology”
2018 – 2nd place at the second stage of the All-Ukrainian competition of student research papers in the specialty “Biology”
2019 – 1st place at the 88th scientific-practical conference of students and young scientists with international participation “INNOVATION IN MEDICINE”
2019 – 1st place at the second stage of the All-Ukrainian competition of student research papers in the specialty “Biology”
2019 – winner of the competition for the best report among young scientists in a poster session
Articles in scopus-indexed journals
2024
- Demianchuk, O., Lylyk, M., Balatskiy, V., Gospodaryov, D., & Bayliak, M. (2024). Alpha-ketoglutarate supplementation in long-lived Drosophila melanogaster: Impact on lifespan and metabolic responses. Archives of insect biochemistry and physiology, 116(1), e22116. https://doi.org/10.1002/arch.22116
- Demianchuk, O., Vatashchuk, M., Gospodaryov, D., Hurza, V., Ivanochko, M., Derkachov, V., Berezovskyi, V., Lushchak, O., Storey, K. B., Bayliak, M., & Lushchak, V. I. (2024). High-fat high-fructose diet and alpha-ketoglutarate affect mouse behavior that is accompanied by changes in oxidative stress response and energy metabolism in the cerebral cortex. Biochimica et biophysica acta. General subjects, 1868(1), 130521. https://doi.org/10.1016/j.bbagen.2023.130521
2023
- Vatashchuk, M. V., Bayliak, M. M., Hurza, V. V., Demianchuk, O. I., Gospodaryov, D. V., & Lushchak, V. I. (2023). Alpha-ketoglutarate partially alleviates effects of high-fat high-fructose diet in mouse muscle. EXCLI journal, 22, 1264–1277. https://doi.org/10.17179/excli2023-6608
- Bayliak, M. M., Demianchuk, O. I., Gospodaryov, D. V., Balatskyi, V. A., & Lushchak, V. I. (2023). Specific and combined effects of dietary ethanol and arginine on Drosophila melanogaster. Drug and chemical toxicology, 46(5), 895–905. https://doi.org/10.1080/01480545.2022.2105863
2022
- Bayliak, M. M., Vatashchuk, M. V., Gospodaryov, D. V., Hurza, V. V., Demianchuk, O. I., Ivanochko, M. V., Burdyliuk, N. I., Storey, K. B., Lushchak, O., & Lushchak, V. I. (2022). High fat high fructose diet induces mild oxidative stress and reorganizes intermediary metabolism in male mouse liver: Alpha-ketoglutarate effects. Biochimica et biophysica acta. General subjects, 1866(12), 130226. https://doi.org/10.1016/j.bbagen.2022.130226
2021
- Bayliak, M. M., Sorochynska, O. M., Kuzniak, O. V., Gospodaryov, D. V., Demianchuk, O. I., Vasylyk, Y. V., Mosiichuk, N. M., Storey, K. B., Garaschuk, O., & Lushchak, V. I. (2021). Middle age as a turning point in mouse cerebral cortex energy and redox metabolism: Modulation by every-other-day fasting. Experimental gerontology, 145, 111182. https://doi.org/10.1016/j.exger.2020.111182
2020
- Bayliak, M. M., Demianchuk, O. I., Gospodaryov, D. V., Abrat, O. B., Lylyk, M. P., Storey, K. B., & Lushchak, V. I. (2020). Mutations in genes cnc or dKeap1 modulate stress resistance and metabolic processes in Drosophila melanogaster. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 248, 110746. https://doi.org/10.1016/j.cbpa.2020.110746