Laboratory of plant micropropagation is focused on research and innovative approaches in the field of micropropagation and plant tissue culture for the needs of agriculture and biomedical research
The Plant Micropropagation Laboratory specializes in the preparation of tissue cultures, micropropagation techniques, and the comprehensive study of plant physiological and biochemical parameters to optimize growth conditions and enhance the production of bioactive compounds.
1. Preparation of Tissue Culture, Micropropagation, and Study of Plant Physiology and Biochemistry
Our lab is equipped to prepare sterile tissue cultures, including surface sterilization of explants and the preparation of culture media. We use advanced micropropagation techniques to grow plants, covering the initiation, multiplication, and rooting stages. Spectrophotometric and chromatographic methods are used to determine antioxidant properties and phytochemical composition of plants, including the analysis of polyphenol, flavonoid, vitamin, chlorophyll, and carotenoid content. We aim to establish optimal growth conditions for these plants to maximize their phytochemical potential.
2. Callus Induction, Enhancement of Flavonoid Content, and Development of Dietary Supplements
We induce callus formation by optimizing phytohormone concentrations in the culture media. Elicitors such as silver nitrate and additional phytohormones are used to enhance the flavonoid content in callus cultures. The dried callus is then developed into dietary supplements. Our cultivation protocols are refined based on thorough analysis to ensure consistent and high yields of bioactive compounds.
3. Data Analysis and Visualization
Data analysis and visualization are conducted using Origin, R, and Python. We interpret the results to understand changes in bioactive compound content and develop scalable processes for producing high-quality medicinal plant materials. This rigorous data analysis allows us to continually optimize our methodologies and outputs.
4. Application and Dissemination
Our findings form a valuable set of preliminary data for future large-scale studies and potential commercial applications. We disseminate our results through scientific publications and presentations, advancing the use of medicinal plants in modern healthcare and phytotherapy. Our goal is to contribute to the broader scientific community and support the integration of medicinal plants into contemporary therapeutic practices.
- Autoclave room
- Working room for preparation of culture media, physiological and biochemical assays
- Room for microclonal propagation with two laminar flow cabinets
- Room for growth of miclocloned plants in the plastic vials with chemically defined media in the standardized conditions
- Room for adaptation of miclocloned plants to growth in the soil
- Spectrophotometer ULAB102
- pH-meters: pH meter 150 MI, portable pH meter
- Class II Biosafety Cabinets “BBP-02” SERIES – 2 units
- Bacteriological air recirculator – 5 units
- Mini-centrifuge “Mini Fuge” – 2 units
- Benchtop 23L autoclave TUTTNAUER 2540EK-B/L
- Industrial autoclave VK-70 for 70 L
- Distiller DE-20
- Water bath 1136D
- Electric lab scales
- CO2 іncubator Thermo Scientific
- Metal racks equipped with lighting – 20 units.
The team at the Plant Micropropagation Laboratory offers technical training for researchers, including the use of microscopes, tissue culture techniques, and data analysis tools. We provide introductions for scientists who want to start working with plant tissue culture models, offer advice for planning experiments, and familiarize them with our equipment. Our staff has extensive experience in plant micropropagation and research, and we are committed to helping you optimize your work with plant tissue cultures. We have extensive experience and facilities for in vitro plant propagation that we used for plants such as banana, blueberry, blackberry, rose, raspberry, elderberry, stevia, paulownia, gynura, rosehip, gooseberry, mint, artichoke, currant, and others.
