Dr. Dmytro Gospodaryov

Dmytro V. Gospodaryov

Associate Professor, Ph. D.
Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University

 

 

CVPublications

І. EDUCATION
Ph.D. thesis

“Protein and lipid oxidation in the yeast Saccharomyces cerevisiae and possible role of catalases in its prevention” (in Ukrainian), done in Department of Biochemistry of Vassyl Stefanyk Precarpathian National University and defended in Scientific Council D 76.05.051 at Fed’kovich Chernivtsi National University (December, 2005); supervisor – D. Sc., Ph.D., Professor Lushchak V.I., Head of Department of Biochemistry of Vassyl Stefanyk Precarpathian National University.

M.Sc. Thesis
“Ecological and physiological parameters of microorganisms by action heavy metals and ultraviolet radiation”, (in Ukrainian), September 2000–May 2001; supervisor: Dr. Gorshinsky B. M., Department of Biochemistry and Experimental Ecology, Biology Faculty, Fed’kovich Chernivtsy National University, Chernivtsi, Ukraine.

II. SCIENTIFIC DEGREES
Doctor of Philosophy, Speciality – Biochemistry.

II. TEACHING EXPERIENCE

Integration of metabolism, special course for masters – lectures and seminars, 2014-2021, 2023-present.
Gene expression, special course for bachelors – lectures, 2008.
Gene expression, special course for bachelors – seminars, 2008.
Cell biology, special course for masters – lectures and seminars, 2006-2010, 2013-2020.
Cell biology, special course for bachelors – lectures and seminars, 2006-2010, 2013-2019.
Cell biology, special course for bachelors – lectures, 2013-2020.
Mathematical methods in biology, a special course for undergraduates and graduate students – 2008-2010, 2013-present.
Structure and functions of biomembranes, special course for bachelors – lectures and seminars, 2013-2020.
Bioenergy, special course for bachelors – lectures and seminars, 2008-2010, 2013-2019.
Virology, general course – lectures, 2007-2009.
Virology, general course – seminars, 2006-2007, 2013-2015.
Algology and micrology, general course – laboratory classes, 2006.
Mycology, a special course for biochemistry students – lectures, 2006.
Mycology, a special course for students majoring in biochemistry – seminars, 2006.
Biochemistry of adaptations, a special course for specialists and masters in biology (specialization – biochemistry) – seminars, 2006.
Biochemistry, general course – laboratory classes, 2005-2006.
Microbiology, general course – laboratory classes, 2005-2006.
Ichthyology, general course – practical classes, 2005.
Hydrobiology, general course – practical classes, 2005.
Biochemistry of adaptations, general course – seminars, 2004-2005.
Molecular biology, general course – seminars, 2004-2006.

IV. RESEARCH EXPERIENCE
2011-2013 Postdoctoral Fellow at FINMIT-Howy Jacobs’ group, Institute of Biomedical Technology, University of Tampere, Finland.
2006-present Senior Research fellow, Department of Biochemistry, Vassyl Stefanyk
Precarpathian National University, Ivano-Frankivsk, Ukraine.
2004-2006 Research fellow, Department of Biochemistry, Vassyl Stefanyk
Precarpathian National University, Ivano-Frankivsk, Ukraine.
2003-2004 Postgraduate student, Department of Biochemistry, Vassyl Stefanyk
Precarpathian National University, Ivano-Frankivsk, Ukraine.
2001-2003 Postgraduate student of Department of Biology, Vassyl Stefanyk
Precarpathian National University, Ivano-Frankivsk, Ukraine.

V. STUDENT SUPERVISION

3rd grade students

2018 Marian Ivanochko
2017 Garidzhuk Svitlana
2008 Mariana Zhdaniuk, Ksenia Koturbash
2007 Tetiana Babiichuk, Olha Velychko, Anastasia Popadynets, Olga Golovchuk
2006 Vasyl Kuzenko, Yulia Lushchak, Sofia Markevych
2005 Svitlana Ninova, Halyna Lesiv

Bachelors

2022 Natalia Nechytailo
2021 Ivan Kornat, Roman Karpenko
2019 Marian Ivanochko
2018 Vitaliy Balatskyi, Svitlana Garidzhuk
2017 Taras Pryimak
2007 Vasyl Kuzenko, Sofia Markevich
2006 Svitlana Ninova

5th grade students

2011 Liliya Kuziuk
2009 Anastasia Popadynets
2008 Vasyl Kuzenko
2005 Liliya Pankiv

Masters

2021-2022 Vasylyna Khrystenko
2020 Marian Ivanochko
2019 Natalia Butenko
2014 Victoria Vivsianyk
2011 Nataliya Perkhulyn
2010 Mariana Zhdaniuk, Ksenia Koturbash
2006 Vasyl Kosar

VI. AWARDS AND GRANTS

FEMS Young Scientist Meeting Grant in order to attend the 18th European Bioenergetics Conference (Lisbon, Portugal)

Grant from Centre for International Mobility (CIMO, Finland), 2011-2012.

Grant for attending in FEBS advanced course “Frontiers in Molecular Biochemistry of Mitochondria” (June 9-17, 2006, Nencki Institute of Experimental Biology, Warsaw, Poland)

VII. CURRENT AREAS OF INTEREST
Gene therapy for cure of mitochondrial diseases
Alternative respiratory chains
Anti-aging pharmaceutical remedies
Mathematical models of aging
Positive and negative role of free radical enzyme inactivation
Role of NADPH in the cell
Melanogenesis in soil bacteria
Iron metabolism in living organisms

VIII. PROFESSIONAL SOCIETIES

Ukrainian Biochemical Society
Ukrainian Society of Cell Biology
Society of Microbiologists of Ukraine

Books and chapters:

  1. Lushchak O., Gospodaryov D., Strilbytska O., Bayliak M. Chapter 5 – Changing ROS, NAD and AMP: A path to longevity via mitochondrial therapeutics. Ed.: U. Çakatay, M. Can Atayik. Advances in Protein Chemistry and Structural Biology. Cambridge, MA: Academic Press, 2023. Vol. 136, 157-196. ISBN: 978-0-443-18886-2, DOI: 10.1016/bs.apcsb.2023.03.005
  2. Lushchak O., Gospodaryov D. Mimetics of caloric restriction, in: Anti-aging drugs: from basic research to clinical practice. Ed.: A.M. Vaiserman, Croydon: Royal Society of Chemistry. 2017, 229-271.
  3. Lushchak V.I., Gospodaryov D.V. Introductory Chapter, in: Oxidative Stress and Diseases, Eds.: V.I. Lushchak & D.V. Gospodaryov, 2012, 8-12, ISBN: 978-953-51-0552-7, DOI: 10.5772/39293.
  4. Gospodaryov D.V., Lushchak V.I., Oxidative Stress: Cause and Consequence of Diseases, in: Oxidative Stress and Diseases, Eds.: V.I. Lushchak & D.V. Gospodaryov D.V., 2012, 13-38, ISBN: 978-953-51-0552-7, DOI: 10.5772/39293.

Articles:

  1. Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses / O. Demianchuk et al. Archives of Insect Biochemistry and Physiology. 2024. Vol. 116, no. 1, URL: https://doi.org/10.1002/arch.22116 (date of access: 27.11.2024).
  2. Life history trade-offs in Drosophila: flies select a diet to maximize reproduction at the expense of lifespan / O. Strilbytska et al. The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences. 2024. URL: https://doi.org/10.1093/gerona/glae057 (date of access: 27.11.2024).
  3. Impact of caloric restriction on oxidative stress and key glycolytic enzymes in the cerebral cortex, liver and kidney of old and middle-aged mice / M. V. Vatashchuk et al. Neuropharmacology. 2024. P. 109859. URL: https://doi.org/10.1016/j.neuropharm.2024.109859 (date of access: 27.11.2024).
  4. High-fat high-fructose diet and alpha-ketoglutarate affect mouse behavior that is accompanied by changes in oxidative stress response and energy metabolism in the cerebral cortex / O. Demianchuket al. Biochimica et Biophysica Acta (BBA) – General Subjects. 2024. Vol. 1868, no. 1. P. 130521. URL: https://doi.org/10.1016/j.bbagen.2023.130521 (date of access: 27.11.2024).
  5. Demianchuk OI, Ivanochko MV, Gospodaryov DV, Bayliak MM. Rhodiola rosea and ferulic acid activate expression of genes related to autophagy and resistance to heat shock in mice of different age. Biotechnologia Acta. 2023; 16(2):15-17. doi: 10.15407/biotech16.02.015
  6. Demianchuk O, Butenko N, Gospodaryov D, Bayliak M. Effects of feeding with non-autoclaved and autoclaved fructose-arginine mixture on stress resistance of Drosophila melanogaster. Journal of Vasyl Stefanyk Precarpathian National University. 2023; 9(4): 15-24. doi: 10.15330/jpnu.9.4.15-24
  7. Demianchuk O, Vatashchuk M, Gospodaryov D, Hurza V, Ivanochko M, Derkachov V, Berezovskyi V, Lushchak O, Storey KB, Bayliak M, Lushchak VI. High-fat high-fructose diet and alpha-ketoglutarate affect mouse behavior that is accompanied by changes in oxidative stress response and energy metabolism in the cerebral cortex. Biochim Biophys Acta Gen Subj. 2023; 1868(1): 130521. doi: 10.1016/j.bbagen.2023.130521
  8. Bayliak MM, Demianchuk OI, Gospodaryov DV, Balatskyi VA, Lushchak VI. Specific and combined effects of dietary ethanol and arginine on Drosophila melanogaster. Drug Chem Toxicol. 2023; 46(5): 895-905. doi: 10.1080/01480545.2022.2105863
  9. Gospodaryov DV, Ballard JWO, Camus MF, DeSalle R, Garvin MR, Richter U. Editorial: Energy-producing organelles and the nucleus: a phenomenal genomic friendship. Front Genet. 2023; 14: 1230032. doi: 10.3389/fgene.2023.1230032
  10. Matskevych V, Kamyshnyi O, Vasylyk VM, Grynovska MB, Lenchuk T, Fishchuk R, Gospodaryov D, Yurkevych I, Strilbytska O, Petakh P, Lushchak O. Morphological prediction of lethal outcomes in the evaluation of lung tissue structural changes in patients on respiratory support with СOVID-19: Ukrainian experience. Pathol Res Pract. 2023; 245:154471. doi: 10.1016/j.prp.2023.154471
  11. Bayliak MM, Gospodaryov DV, Lushchak VI. Homeostasis of carbohydrates and reactive oxygen species is critically changed in the brain of middle-aged mice: Molecular mechanisms and functional reasons. BBA Adv. 2023; 3: 100077. doi: 10.1016/j.bbadva.2023.100077
  12. Bayliak MM, Vatashchuk MV, Gospodaryov DV, Hurza VV, Demianchuk OI, Ivanochko MV, Burdyliuk NI, Storey KB, Lushchak O, Lushchak VI. High fat high fructose diet induces mild oxidative stress and reorganizes intermediary metabolism in male mouse liver: Alpha-ketoglutarate effects. Biochim Biophys Acta Gen Subj. 2022; 1866(12): 130226. doi: 10.1016/j.bbagen.2022.130226
  13. Semaniuk UV, Gospodaryov DV, Strilbytska OM, Kucharska AZ, Sokół-Łętowska A, Burdyliuk NI, Storey KB, Bayliak MM, Lushchak O. Chili-supplemented food decreases glutathione-S-transferase activity in Drosophila melanogaster females without a change in other parameters of antioxidant system. Redox Rep. 2022; 27(1): 221-229. doi: 10.1080/13510002.2022.2123884
  14. Semaniuk UV, Gospodaryov DV, Strilbytska OM, Kucharska AZ, Sokół-Łętowska A, Burdyliuk NI, Storey KB, Bayliak MM, Lushchak O. Chili pepper extends lifespan in a concentration-dependent manner and confers cold resistance on Drosophila melanogaster cohorts by influencing specific metabolic pathways. Food Funct. 2022; 13(15): 8313-8328. doi: 10.1039/d2fo00930g
  15. Bayliak MM, Sorochynska OM, Kuzniak OV, Gospodaryov DV, Demianchuk OI, Vasylyk YV, Mosiichuk NM, Storey KB, Garaschuk O, Lushchak VI. Middle age as a turning point in mouse cerebral cortex energy and redox metabolism: Modulation by every-other-day fasting. Exp Gerontol. 2021; 145: 111182. doi: 10.1016/j.exger.2020.111182.
  16. Bayliak MM, Demianchuk OI, Gospodaryov DV, Abrat OB, Lylyk MP, Storey KB, Lushchak VI. Mutations in genes cnc or dKeap1 modulate stress resistance and metabolic processes in Drosophila melanogaster. Comp Biochem Physiol A Mol Integr Physiol. 2020; 248: 110746. doi: 10.1016/j.cbpa.2020.110746.
  17. Yurkevych IS, Gray LJ, Gospodaryov DV, Burdylyuk NI, Storey KB, Simpson SJ, Lushchak O. Development of fly tolerance to consuming a high-protein diet requires physiological, metabolic and transcriptional changes. Biogerontology. 2020; 21 (5): 619-636. doi: 10.1007/s10522-020-09880-0.
  18. Gospodaryov DV, Strilbytska OM, Semaniuk UV, Perkhulyn NV, Rovenko BM, Yurkevych IS, Barata AG, Dick TP, Lushchak OV, Jacobs HT. Alternative NADH dehydrogenase extends lifespan and increases resistance to xenobiotics in Drosophila. Biogerontology. 2020; 21 (2): 155-171. doi: 10.1007/s10522-019-09849-8.
  19. Sorochynska OM, Bayliak MM, Gospodaryov DV, Vasylyk YV, Kuzniak OV, Pankiv TM, Garaschuk O, Storey KB, Lushchak VI. Every-other-day feeding decreases glycolytic and mitochondrial energy-producing potentials in the brain and liver of young mice. Front Physiol. 2019; 10: 1432. doi: 10.3389/fphys.2019.01432.
  20. Bayliak MM, Lylyk MP, Gospodaryov DV, Kotsyubynsky VO, Butenko NV, Storey KB, Lushchak VI. Protective effects of alpha-ketoglutarate against aluminum toxicity in Drosophila melanogaster. Comp Biochem Physiol C Toxicol Pharmacol. 2019; 217: 41-53. doi: 10.1016/j.cbpc.2018.11.020.
  21. McDonald AE, Gospodaryov DV. Alternative NAD(P)H dehydrogenase and alternative oxidase: Proposed physiological roles in animals. Mitochondrion. 2019; 45: 7-17. doi: 10.1016/j.mito.2018.01.009.
  22. Semaniuk UV, Gospodaryov DV, Feden’ko KM, Yurkevych IS, Vaiserman AM, Storey KB, Simpson SJ, Lushchak O. Insulin-like peptides regulate feeding preference and metabolism in Drosophila. Front Physiol. 2018; 9: 1083. doi: 10.3389/fphys.2018.01083.
  23. Lushchak OV, Gospodaryov DV, Yurkevych IS, Storey KB. Oxidized lipids did not reduce lifespan in the fruit fly, Drosophila melanogaster. Arch Insect Biochem Physiol. 2016; 91 (1): 52-63. doi: 10.1002/arch.21308.
  24. Gospodaryov D. Underinvestigated roles of glucose-6-phosphate dehydrogenase. Journal of Vasyl Stefanyk Precarpathian National University. 2015; 2 (1): 25-37.
  25. Rovenko BM, Kubrak OI, Gospodaryov DV, Perkhulyn NV, Yurkevych IS, Sanz A, Lushchak OV, Lushchak VI. High sucrose consumption promotes obesity whereas its low consumption induces oxidative stress in Drosophila melanogaster. J Insect Physiol. 2015; 79: 42-54. doi: 10.1016/j.jinsphys.2015.05.007.
  26. Rovenko BM, Kubrak OI, Gospodaryov DV, Yurkevych IS, Sanz A, Lushchak OV, Lushchak VI. Restriction of glucose and fructose causes mild oxidative stress independently of mitochondrial activity and reactive oxygen species in Drosophila melanogaster. Comp Biochem Physiol A Mol Integr Physiol. 2015; 187: 27-39. doi: 10.1016/j.cbpa.2015.04.012.
  27. Rovenko BM, Perkhulyn NV, Gospodaryov DV, Sanz A, Lushchak OV, Lushchak VI. High consumption of fructose rather than glucose promotes a diet-induced obese phenotype in Drosophila melanogaster. Comp Biochem Physiol A Mol Integr Physiol. 2015; 180: 75-85. doi: 10.1016/j.cbpa.2014.11.008.
  28. Gospodaryov DV, Lushchak OV, Rovenko BM, Perkhulyn NV, Gerards M, Tuomela T, Jacobs HT. Ciona intestinalis NADH dehydrogenase NDX confers stress-resistance and extended lifespan on Drosophila. Biochim Biophys Acta. 2014; 1837 (11): 1861-1869. doi: 10.1016/j.bbabio.2014.08.001.
  29. Lushchak OV, Gospodaryov DV, Rovenko BM, Yurkevych IS, Perkhulyn NV, Lushchak VI. Specific dietary carbohydrates differentially influence the life span and fecundity of Drosophila melanogaster. J Gerontol A Biol Sci Med Sci. 2014; 69 (1): 3-12. doi: 10.1093/gerona/glt077.
  30. Gospodaryov DV, Yurkevych IS, Lushchak OV, Lushchak VI. Correction: Lifespan extension and delay of age-related functional decline caused by Rhodiola rosea depends on dietary macronutrient balance. Longevity & Healthspan 2013; 2(1):12. doi:10.1186/2046-2395-2-12.
  31. Gospodaryov DV, Yurkevych IS, Jafari M, Lushchak VI, Lushchak OV. Lifespan extension and delay of age-related functional decline caused by Rhodiola rosea depends on dietary macronutrient balance. Longevity & Healthspan 2013; 2(1):5. doi: 10.1186/2046-2395-2-5.
  32. Lushchak OV, Gospodaryov DV, Rovenko BM, Glovyak AD, Yurkevych IS, Klyuba VP, Shcherbij MV, Lushchak VI. Balance between macronutrients affects life span and functional senescence in fruit fly Drosophila melanogaster. J Gerontol A Biol Sci Med Sci. 2012; 67(2): 118-125. doi: 10.1093/gerona/glr184.
  33. Lushchak OV, Rovenko BM, Gospodaryov DV, Lushchak VI. Drosophila melanogaster larvae fed by glucose and fructose demonstrate difference in oxidative stress markers and antioxidant enzymes of adult flies. Comp Biochem Physiol A Mol Integr Physiol. 2011; 160(1):27-34. doi: 10.1016/j.cbpa.2011.04.019.
  34. Gospodaryov D. Lushchak V. Some properties of melanin produced by Azotobacter chroococcum and its possible application in biotechnology. Біотехнологія : наук. журн. 2011; 4 (2): 61-69.
  35. Bayliak M, Gospodaryov D, Semchyshyn H, Lushchak V. Inhibition of catalase by aminotriazole in vivo results in reduction of glucose-6-phosphate dehydrogenase activity in Saccharomyces cerevisiae cells. Biochemistry (Mosc). 2008; 73 (4): 420-426. DOI: 10.1134/S0006297908040068.
  36. Lushchak VI, Gospodaryov DV. Catalases protect cellular proteins from oxidative modification in Saccharomyces cerevisiae. Cell Biol Int. 2005; 29 (3): 187-192. doi:10.1016/j.cellbi.2004.11.001.
  37. Hospodar’ov DV, Lushchak VI. [Iron metabolism in the yeast]. Ukr Biokhim Zh. 2005; 77 (3): 5-19. Ukrainian.
  38. Hospodar’ov DV, Baĭliak MM, Lushchak VI. [Free radical inactivation of glucose-6-phosphate dehydrogenase in Saccharomyces cerevisiae in vitro]. Ukr Biokhim Zh. 2005; 77 (1): 58-64. Ukrainian.
  39. Hospodar’ov DV, Mandryk SIa, Lushchak VI. [The role of catalases in protection of proteins against oxidation in Saccharomyces cerevisiae utilizing ethanol as a carbon source]. Ukr Biokhim Zh. 2005; 77 (2): 162-165. Ukrainian.
  40. Hospodar’ov DV, Lushchak VI. [Effect of iron ions on the antioxidant enzyme activities in yeast Saccharomyces cerevisiae]. Ukr Biokhim Zh. 2004; 76(6): 100-105. Ukrainian.

Selected abstracts

  1. Gospodaryov D., Yurkevych I., Semanyuk U., Burdyliuk N., Strilbytska O., Lushchak O. Plant preparations and drugs to extend lifespan in Drosophila. Drosophila in experimental genetics and biology: Abstract book of the 8th International conference (Ivano-Frankivsk, June 27–29, 2023). Ivano-Frankivsk, 2023. P. 32.
  2. Gospodaryov D, Lylyk M, Demianchuk O, Sitko M, Yurchak T, Bayliak M. Deficiency in Nrf2 and Keap1 delays development and modulates metabolic processes in Drosophila melanogaster, in: 26th European Drosophila Research Conference (5th-8th September 2019, EPFL, Switzerland). Program & Abstract Book: 196-197.
  3. Gospodaryov D, Balatskiy V, Bayliak M. Bioenergetic basis for the effects of arginine and alpha-ketoglutarate on lifespan. in: Materials of 20th European Bioenergetics Conference, Budapest, Hungary, August 25-30, (2018) Abstracts. Biochim Biophys Acta. 2018. 1859 (Suppl.): e59. doi: 10.1016/j.bbabio.2018.09.177.
  4. Gospodaryov D, Perkhulyn N, Rovenko B, Strilbytska O, Semanyuk U, Lushchak O. Alternative NADH dehydrogenase from ascidian Ciona intestinalis prolongs lifespan of fruit fly and confers it resistance to inorganic and organic toxicants, in: Materials of 19th European Bioenergetics Conference, Riva del Garda, Italy, July 2-7, (2016) Abstracts. Biochim Biophysica Acta. 2016; 1857 (Suppl.): e38. doi: 10.1016/j.bbabio.2016.04.115.
  5. Gospodaryov D, Bayliak M, Lushchak O, Semchyshyn H, Lushchak V. Oxidative stress at a crossroads of adaptation, in: International Symposium on Cell Biology jointly with 4rd Ukrainian Congress of Cell Biology (17th-20th September 2014, Uzhgorod, Ukraine). Abstract book: 48.
  6. Gospodaryov DV, Rovenko BM, Perkhulyn NV, Jacobs HT. Properties of ascidian alternative NADH dehydrogenase.in: Materials of 18th European Bioenergetics Conference, Lisbon, Portugal, July 12-17, (2014) Abstracts. Biochim Biophys Acta. 2014 Jul;1837(Suppl.):e71-e72. doi: 10.1016/j.bbabio.2014.05.013.
  7. Gospodaryov D.V., Jacobs H.T. Expression of ascidian alternative NADH dehydrogenase in Drosophila. in: Materials of 38th FEBS Congress “Mechanisms in Biology”, Saint Petersburg, Russia, July 6-11, (2013) Main Symposia and Workshops. FEBS Journal 280 (Suppl. 1) (2013) 261-262. DOI: 10.1111/febs.12340.

Blogs

1. Gospodaryov D., Lushchak O. Will caloric restriction help you live longer? March 20th, 2014. OUPBlog. http://blog.oup.com/2014/03/caloric-restriction-carbohydrates-diet-longevity/