Oleksandra Abrat

Oleksandra ABRAT

Associate Professor, Ph.D.

Deputy Dean for Academic Affairs
Faculty of Natural Sciences

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 SCOPUS https://www.scopus.com/authid/detail.uri?authorId=8886483400

ORCID https://orcid.org/0000-0003-4477-3032

WEB OF SCIENCE https://www.webofscience.com/wos/author/record/1072482

Goggle Scholar https://scholar.google.com/citations?hl=uk&user=NvDgScYAAAAJ

І. ADDRESS

Work address: Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenko str., Ivano-Frankivsk, 76018, Ukraine

E-mail: oleksandra.abrat@pnu.edu.ua

Phone:  +38(0342)596171

ІІ. PERSONAL INFORMATION

Ukrainian, married, born May 16, 1983

Languages: Ukrainian, English (professional working proficiency)

ІІІ. EDUCATION

Ph.D. thesis

Mechanisms of defense of yeast Saccharomyces cerevisiae against weak acid stress [in Ukrainian], defended in Scientific Council D 76.06.051 at Yurij Fed’kovych Chernivtsi National University, Ukraine (October, 2009). Supervisor – D. Sc., Ph.D., Prof Lushchak V.I., Head of Department of Biochemistry PNU.

M.Sc. Thesis

Role catalases in protection of yeast Saccharomyces cerevisiae from oxidative stress [in Ukrainian], September 2004-May 2005. Supervisor – D. Sc., Ph.D., Prof Lushchak V.I., Head of Department of Biochemistry PNU.

ІV. TEACHING EXPERIENCE

  • 2018-present Associate Professor, Biochemistry and Biotechnology Dept., PNU.
  • 2007-2017 Assistant of Professor, Biochemistry Dept., PNU.

Сourses:

  • Immunology, general course (lectures & practice, 2018 – present, PNU);
  • Microbiology, general course (lectures & practice, 2008 – present, PNU); 
  • Endocrinology, special course for bachelors in biochemistry (lectures & practice, 2023 – present, PNU); 
  • Basic Biochemistry (lectures & practice, 2017 – present, PNU); 
  • Biochemistry with the basics of microbiology and virology, general course (lectures & practice, 2021-2023, PNU);
  • Molecular basis of immunity, special course for masters in biochemistry (lectures & practice, 2021, PNU);
  • Molecular immunology, special course for bachelors in biochemistry (lectures & practice, 2022, PNU);
  • Molecular endocrinology, special course for bachelors in biochemistry (lectures & practice, 2018 – 2022, PNU); 
  • Biochemistry, general course (lectures & practice, 2018 – present, PNU); 
  • Virology, general course (lectures & seminars, 2008–2011 2016 – present, PNU);
  • Biochemistry of adaptations, masters in biology (seminars, 2007–2008, PNU);
  • Molecular biology, general course (seminars, 2007–2008, PNU)

   V. PROFESSIONAL EXPERIENCE / TRAINING / SCHOLARSHIP:

  • 2022-present: Associate Professor in the Grant program from the German academic exchange service DAAD
  • 2018–present: Associate Professor, Department of Biochemistry and Biotechnology PNU;
  • 2020-2023: Associate researcher in the project “Personalized prevention tools in obesity and diabetes – a joint Romanian-Ukrainian Programme of health education (PrePOD)” (EMS ENI Code 2SOFT / 4.1 / 56);
  • 2019: Research Assistant, Kielanowski Institute of Animal Physiology and Nutrition, Jablonna, Poland.
  • 2009–2017: Research Assistant, Department of Biochemistry and Biotechnology PNU;
  • 2012-2013: Head of Laboratory, Department of Biochemistry and Biotechnology PNU;
  • 2005–2008: Postgraduate student, Department of Biochemistry, PNU;
  • 2007:          Laboratory of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.

Training / scholarship

  • Theoretical training (total credits 2,3 ECTS) “MOLECULAR BIOLOGY, BIOPHYSICS, BIOENERGETICS AND PHYSIOLOGY in course LIFE SCIENCES“. The course was taught German academic exchange service DAAD within the framework of the project online course Ukraine digital: Ensuring academic success in times of crisis from March to May, 2024.
  • Theoretical training (total credits 1 ECTS) “Immunology“. The course was taught German academic exchange service DAAD within the framework of the project online course Ukraine digital: Ensuring academic success in times of crisis from October to December, 2023.
  • Theoretical training (total credits 1 ECTS) “Mastering online teaching of life sciences“. The course was taught German academic exchange service DAAD within the framework of the project online course Ukraine digital: Ensuring academic success in times of crisis from February to March, 2023.
  • Theoretical training (total credits 6 ECTS) “Nobel Laureates: Researching the Experience and Professional Achievements for Successful Personality Development and World Transformation” (2022, The International Historical Biographical Institute);
  • Internship: “Testing the stability of enteric coating delay release ALLN-346 tablets along gastrointestinal tract in fed and fasted state, ALLN-346 PK study in the gut” (2019, Lund University, Lund, Sweden);
  • PolLASA courses on proper breeding, maintenance, and usage of laboratory animals (2019, Polish laboratory animal science association, Warsaw, Poland);
  • Theoretical training (total credits 2 ECTS) “Perspectives in Biomedicine with a Focus on Cancer Immunotherapy” (2019, DAAD, Ivano-Frankivsk, Ukraine);
  • The Queen Jadwiga Fund (2013, Jagiellonian University, Krakow, Poland);
  • The Queen Jadwiga Fund (2007, Jagiellonian University, Krakow, Poland)

VI.CURRENT AREAS OF INTEREST

  • Post traumatic stress disorder (mice)
  • Metabolic syndrome and obesity (fruit flies, mice, humans)
  • Antioxidant, adaptogenic, antiglycemic, stress-protective, and geroprotective effects of natural products und drugs (fruit flies, mice)
  • Oxidative stress and antioxidants (E.coli, yeast)

VII. SKILLS

Preparation of tissue and cell extracts, enzyme activities assays, measurement of content of proteins, carbohydrates and lipids; measurement of major marker of oxidative stress, blood chemistry and blood enzymes tests; leukocyte formula and enzyme-linked immunosorbent assay (ELISA); fluorometric analysis of fluorescein extrusion; light microscopy, cultivation of microorganisms, isolation of microbial cultures, study of physiologic-biochemical properties of microorganisms.

 VIII. PROFESSIONAL SOCIETIES

  • Ukrainian Biochemical Society
  • Ukrainian Society of Cell Biology
  • Society of Microbiologists of Ukraine

OTHER ACTIVITIES

  • Jury member of National Biological Olympiads
  • Jury member of National Tournament for Young Biologists
  • Jury member of the Small Academy of Sciences research competition
  • Activity manager of Organizing Committee and Lecturer at Carpathian Summer School in Biochemistry held annually at Department of Biochemistry and Biotechnology, PNU
  • Member of Organizing Committee and Lecturer at Autumn School for Young Biochemists held annually at Department of Biochemistry and Biotechnology, PNU.

IX. INTERNATIONAL GRANTS

Grant program from the German academic exchange service DAAD “Ukraine digital: Ensuring academic success in times of crisis (2022, 2023, 2024)” to support the education of Ukrainian students during the war. Blended course “Integrative Life Sciences” for Ukrainian biology students.

Сross-border project ”Personalized prevention tools in obesity and diabetes – a joint Romanian-Ukrainian Programme of health education (PrePOD)“ (2020-2023). Grant 2SOFT / 4.1 / 56.  – Research associate, http://www.prepod.org.ua/en/home_en/

X. PUBLICATIONS

  • Articles – 18
  • Abstracts – 27
  • Teaching works – 26

LIST OF PUBLICATIONS

Monograph chapters:

  1. Bayliak, Maria M., and Oleksandra B. Abrat. “Role of Nrf2 in Oxidative and Inflammatory Processes in Obesity and Metabolic Diseases.” Nrf2 and its Modulation in Inflammation. Springer, Cham. – 2020. –P.153–187.

Review an experimental articles in Q1 and Q2 journals:

  1. Lushchak, V. I., Covasa, M., Abrat, O. B., Mykytyn, T. V., Tverdokhlib, I. Z., Storey, K. B., & Semchyshyn, H. (2023). Risks of obesity and diabetes development in the population of the Ivano-Frankivsk region in Ukraine. EXCLI Journal, 22, 1047-1054.
  2. Bayliak, M. M., Demianchuk, I., Gospodaryov, D. V., Abrat, O. B., Lylyk, M. P., Storey, K. B., & Lushchak, V. I. (2020). Mutations in genes cnc or dKeap1 modulate stress resistance and metabolic processes in Drosophila melanogaster. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 248, 110746.
  3. Interplay between diet-induced obesity and oxidative stress: Comparison between Drosophila and mammals / Bayliak, M. M., Abrat, O., Storey, J. M., Storey, K. B., & Lushchak, V. I.// Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. – 2019. – Vol. 228, P. 12–18.
  4. High amylose starch consumption induces obesity in Drosophila melanogaster and metformin partially prevents accumulation of storage lipids and shortens lifespan of the insects / Abrat Oleksandra B., Storey Janet M., Storey Kenneth B., Lushchak Volodymyr I. // Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. – 2018. – Vol. 215, P. 55–62.
  5. Аcetate but not propionate induces oxidative stress in bakers’ yeast Saccharomyces cerevisiae / Semchyshyn H., Abrat O., Miedzobrodzki J., Inoue Y., Lushchak V. // Red. Report. – 2011. – Vol. 16, N 1. – P. 1–9.
  6. Pdr12p-dependent and -independent fluorescein extrusion from baker’s yeast cells / V. Lushchak, Abrat, J. Międzobrodzki, H. Semchyshyn // Acta biochimіca polonica. – 2008. – Vol. 55, N 3. – P. 595–601.
  7. Acid stress in yeast Saccharomyces cerevisiae / Abrat O.B., Semchyshyn H.M., Lushchak V.I. [in Ukrainian] // Ukr. Biochem. J. 2008. 80 (6), P. 19–31.
  8. Fluorescein transport and antioxidant systems in the yeast Saccharomyces cerevisiae under acid stress / Abrat O.B., Semchyshyn H.M., Międzobrodzki J., Lushchak V.I. [in Ukrainian] // Ukr. Biochem. J. 2008. 80 (3), P. 70-77.
  9. Acid stress increases the activity of superoxide dismutase and catalase in the yeast Saccharomyces cerevisiae / Abrat O., Semchyshyn H., Lushchak V. [in Ukrainian] // Ukr. Biochem. J. 2007. 79 (2), P. 17-23.
  10. Survival and antioxidant defense of the yeast Saccharomyces cerevisiae under starvation and oxidative stress / Bailyak M., Abrat O., Semchyshyn H., Lushchak V. [in Ukrainian] // Ukr. Biochem. J. 2005. 77 (2), P. 162-165.
  11. Survival and antioxidant defense of Esherichia coli in response to alloxan exposure / Abrat O., Semchyshyn H., Lushchak V. [in Ukrainian] // Ukr. Biochem. J. 2005. 77 (2), P. 123-129.

Other articles:

Bayliak M., Abrat O., Vorochylo K. (2023). The content of biologically active substances and total antioxidant activity of black tea drinks and decoctions of medicinal plants fermented with Kombucha. Journal of Vasyl Stefanyk Precarpathian National University. 10, (Dec. 2023), 20-33. https:// doi: 10.15330/jpnubio.10.20-33.

Bayliak M., Abrat O., Shmihel H., Lushchak V. and Shvadchak V. (2023). Interuniversity Online Courses as Possible Approach to Improve Teaching During Crisis: a Ukrainian Case Study. Journal of Vasyl Stefanyk Precarpathian National University. 10, 1 (Apr. 2023), 49-60.

 Abrat O., Diduch J. Influence of dietary yeast restriction on pathological changes in the body of the fruit fly under high consumption of amylose starch // Ukrainian journal of medicine, biology and sport – 2017. – Vol.  4,  N 6. – P. 148–154.

Abrat Influence of amylose starch on development and lifespan of fruit fly Drosophila melanogaster // Journal of Vasyl Stefanyk Precarpathian National University. Vol. 2, No. 1 (2015), 100–106.

Effect of alloxan and hydrogen peroxide on yeast Saccharomyces cerevisiae survival / Abrat O., Markovych O., Lohaza L. [in Ukrainian] // Newsletter of Precarpathian University. Ivano-Frankivsk. P. 5-9.

Effect of different oxygen levels on the growth of Esherichia coli strains MC4100, GS071 and GS047 / Demyanchuk Yu., Abrat O. [in Ukrainian] // Newsletter of Precarpathian University. Ivano-Frankivsk. P. 130-137.