Dr. Volodymyr Shvadchak

Volodymyr V. SHVADCHAK

e-mail                        volodymyr.shvadchak@pnu.edu.ua

Date of Birth:          January, 2, 1983 (Ivano-Frankivsk, Ukraine)

 

Present employment

Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine (since August 2021).

Education

2005-2009 PhD in life science, Faculty of Pharmacy of Strasbourg University, France.

Work ”Two-color fluorescent dyes for monitoring peptide interactions: application to retroviral proteins” was performed in Biophysical Lab under the supervision of Prof. Yves Mély and Dr. Hugues de Rocquigny and defended on the 20^th April 2009.

2000-2005 M. Sc. in Organic Chemistry, Kyiv Taras Shevchenko National University (Ukraine)

Previous positions

Feb 2015 -Jul 2021		Post doc/deputy group leader at the Institute of Organic Chemistry and Biochemistry, Prague (IOCB Prague) (https://www.uochb.cz/en) Research topic: Inhibition of α-synuclein fibrillization.
Feb 2014 -Feb 2015		Post doc in the Nanoscale Biophysics group headed by Dr. Vinod Subramaniam in AMOLF (Atomic and Molecular Physics) institute in Amsterdam (https://amolf.nl) Research topic: Kinetics mechanism of α-synuclein fibrillization.
Sep 2012 -Feb 2014		Post doc in the Nanobiophysics group of University of Twente (Enschede, The Netherlands) headed by Dr. Vinod Subramaniam and Dr. Mireille Claessens (https://www.utwente.nl/tnw/nbp/) Research topic: Conformation of α‑synuclein on membranes and in oligomers.
Aug 2009 -Aug 2012		Post doc in the Laboratory of Cellular Dynamics headed by Dr. Thomas Jovin in Max Planck Institute for Biophysical Chemistry (Göttingen, Germany) (http://www.mpibpc.mpg.de/jovin) Research topic: Interaction of α-synuclein with membranes.
	Awards, Fellowships, Funded Projects

–    2018 Otto Wichterle Award from Czech Academy of Sciences

–    2017 Junior research grant from Czech Science Foundation (GAČR 18-06255Y, “New strategy for inhibition of amyloid fibril formation”)

–    2015 Postdoctoral award for the intrinsically disordered protein subgroup of Biophysical society (work                 “Fibril breaking accelerates α-synuclein fibrillization”)

–    2010 Marie Curie postdoctoral fellowship (Detection of membrane-interacting cytotoxic amyloid                 intermediates with novel fluorescent probes)

–    2009 PhD thesis prize in life sciences domain (“Vie et Santé”) from Strasbourg biological Society

–    2007 Eiffel Fellowship (‘Bourse d’excellence’ from French government) for PhD work

Research activities in the field of Parkinson’s Disease

        I started my work in the field of biophysics of amyloidogenic protein α-synuclein in 2009 during my first postdoctoral training in the lab of Thomas Jovin.  I was investigating the membrane binding of this protein and found dependence of its conformation on the protein density on model membranes.

        When I moved to the Vinod Subramaniam’s group I shifted my research interest and focused on properties and conformation changes of α‑synuclein upon binding to membranes and on oligomeric intermediates of α‑synuclein amyloid fibril formation. The main result of this work was finding of a short domain in α-synuclein sequence that determines equilibrium between membrane-bound helical conformation and formation of amyloid fibrils. (Shvadchak and Subramaniam, Biochemistry, 2014)

        In AMOLF institute I worked on the mechanism of amyloid fibril growth studying the binding of α‑synuclein monomers to the ends of amyloid fibrils using biophysical and structural methods.  The main result of this work was determination of the mechanism of autocatalytic reaction acceleration and quantitative description of the α-synuclein fibril formation kinetics. (Shvadchak et al, J.Phys Chem.B, 2015)

In 2015, I joined a new group established at the IOCB and focused my research on the development of inhibitors of α-synuclein fibrillization based on my expertize in the mechanistic aspects of this process. (Kyriukha et al, J Med Chem, 2019; Shvadchak et al, Angew Chem, 2018)

In 2021, I received a teaching position at Vasyl Stefanyk Precarpathian National University (Ukraine) where I am going to continue development of α-synuclein fibrillization inhibitors with stronger attention to the delivery and activity in animal models.

	ORCiD: 0000-0001-8302-8073
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	ResearchGate
	Scopus Author ID: 13411269400
	Web of Science ResearcherID: E-1475-2017

53 Articles   H-index 27

Times cited 1583, Without self-citations 1436

2024

Galkin, M., Priss, A., Kyriukha, Y., and Shvadchak, V. (2024) Navigating α-Synuclein Aggregation Inhibition: Methods, Mechanisms, and Molecular Targets. Chem. Rec. 24, doi: 10.1002/tcr.202300282

2023

Giordano, L., Shvadchak, V. V.,Arrupe, N., Falomir Lockhart, L. J., Sánchez, V. M., Jovin, T. M. (2023) Tuning of environment-sensitive 3-hydroxychromone fluorophores based on strong donor substituents in positions 2 or 7. Dyes and Pigments, 218, 111479

Galkin, M., Topcheva, O., Priss, A, Borisova, T., Shvadchak, V. V. (2023) Dopamine-Induced Oligomers of α-Synuclein Inhibit Amyloid Fibril Growth and Show No Toxicity. ACS Chem Neurosci, 14 (11), 2027-2034.

2022
Poryvai, A., Galkin, M., Shvadchak, V., Slanina, T. (2022) Red-shifted water-soluble BODIPY photocages for visualisation and controllable cellular delivery of signaling lipids, Angew. Chem. doi: 10.1002/anie.202205855
2021
Galkin, M., Priss, A., Topcheva, O., Yushchenko, D. A. and Shvadchak, V. V. (2021) FRET-based assay for intracellular evaluation of α-synuclein aggregation inhibitors, J Neurochem, 159, 901-12

Kurochka, A. S., Yushchenko, D. A., Bour, P., Shvadchak, V. V. (2021) Influence of lipid membranes on α synuclein aggregation, ACS Chem Neurosci, 12, 825-830.

Priss, A., Afitska, K., Galkin, M., Yushchenko, D. A., Shvadchak, V. V., (2021) Rationally designed protein-based inhibitor of α-synuclein fibrillization in cells, J Med Chem, 64, 6827-6837

Gaur, P., Galkin, M., Kurochka, A.,Ghosh, S.,Yushchenko, D. A., Shvadchak, V. V., (2021) Fluorescent probe for selective imaging of α-synuclein fibrils in living cells, ACS Chem Neurosci, 12 , 1293-1298
2020
Afitska, K., Priss, A., Yushchenko, D. A., Shvadchak, V. V. (2020) Structural optimization of inhibitors of α synuclein fibril growth: affinity to the fibril end as a crucial factor, J Mol Biol 432, 967-977.

Gaur, P., Galkin, M., Hauke, S., Redkin, R., Barnes, C., Shvadchak, V. V., Yushchenko, D. A. (2020). Reversible spatial and temporal control of lipid signaling. Chem Commun (Camb), 56, 10646-10649.

Sysoiev, D., Prochazkova, E., Semenenko, A., Pohl, R., Shishkina, S., Klepetarova, B., Shvadchak, V, Yushchenko, D. A. (2020). Di(benzothienyl)cyclobutenes: toward strained photoswitchable fluorophores. ChemPlusChem, 85, 2084-2092.
2019
Kyriukha, Y.A., Afitska, K., Kurochka, A.S., Sachan, S., Galkin, M., Yushchenko, D.A., Shvadchak, V.V.¬ (2019) α Synuclein dimers as potent inhibitors of fibrillization J Med Chem 62, 10342-10351.

Gaur, P., Kucherak, O.A., Ermakova, Y.G., Shvadchak, V.V., Yushchenko, D.A. (2019) Nitrobenzyl-based fluorescent photocages for spatial and temporal control of signalling lipids in cells, Chem Commun (Camb) 55, 12288-12291.

Afitska, K., Fucikova, A., Shvadchak, V. V., Yushchenko, D. A. (2019). α-Synuclein aggregation at low concentrations. Biochim Biophys Acta Proteins Proteom 1867, 701-709.
2018
Kyriukha, Y., Kucherak, O., Yushchenko, T., Shvadchak, V., Yushchenko, (2018) 3-Hydroxybenzo[g]chromones: Fluorophores with red-shifted absorbance and highly sensitive to polarity emission. Sensors & Actuators 265, 691-98

Shvadchak, V., Afitska, K., Yushchenko, D. (2018) Inhibition of α-synuclein amyloid fibril elongation by blocking fibril ends. Angew Chem 57, 5690-94.

Park, K., Kuo, Y., Shvadchak, V., Ingargiola, A., Dai, X., Hsiung, L., Kim, W., Zhou, H., Zou, P., Levine, A. J., Li, J., Weiss, S. (2018). Membrane insertion of- and membrane potential sensing by- semiconductor voltage nanosensors: Feasibility demonstration. Sci Adv 4, e1601453.

Shvadchak, V., Zgheib, S., Basta, B., Humbert, N., Langedijk, J., Morris, M. C., Ciaco, S., Maskri, O., Darlix, J. L., Mauffret, O., Fosse, P., Real, E. & Mely, Y. (2018). Rationally designed peptides as efficient inhibitors of nucleic acid chaperone activity of HIV-1 nucleocapsid protein. Biochemistry 57, 4562-4573.

Kucherak, O. A., Shvadchak, V. V., Kyriukha, Y. A. & Yushchenko, D. A. (2018). Synthesis of a Fluorescent Probe for Sensing Multiple Protein States. Eur J Org Chem, 5155-5162.
2017
Afitska, K., Fucikova, A., Shvadchak, V. V., Yushchenko, D. A. (2017). Modification of C-terminus provides new insights into the mechanism of α-synuclein aggregation. Biophy J 113, 2182-91.

Semerdzhiev, S. A., Shvadchak, V. V., Subramaniam, V., Claessens, M. (2017). Non-uniform self-assembly: On the anisotropic architecture of alpha-synuclein supra-fibrillar aggregates. Sci Rep 7, 7699.

Shvadchak, V. V., Kucherak, O., Afitska, K., Dziuba, D., Yushchenko, D. A. (2017). Environmentally sensitive probes for monitoring protein-membrane interactions at nanomolar concentrations. Biochim Biophys Acta 1859, 852-59.
2016
Zamotaiev, O. M., Shvadchak, V., Sych, T. P., Melnychuk, N. A., Yushchenko, D., Mely, Y., Pivovarenko, V. G. (2016). Environment-sensitive quinolone demonstrating long-lived fluorescence and unusually slow excited-state intramolecular proton transfer kinetics. Methods Appl Fluoresc, 4, 034004.
2015
Shvadchak, V. V., Claessens, M. M., Subramaniam, V. (2015). Fibril Breaking Accelerates alpha-Synuclein Fibrillization. J Phys Chem B, 119, 1912-18.
2014
Zamotaiev, O. M., Postupalenko, V. Y., Shvadchak, V. V., Pivovarenko, V. G., Klymchenko, A. S., Mely, Y. (2014) Monitoring penetratin interactions with lipid membranes and cell internalization using a new hydration-sensitive fluorescent probe. Org Biomol Chem, 12, 7036-44.

Shvadchak, V. V., Subramaniam, V. (2014) A four-amino acid linker between repeats in the alpha-synuclein sequence is important for fibril formation. Biochemistry 53, 279-81.
2013
Postupalenko, V. Y., Zamotaiev, O. M., Shvadchak, V. V., Strizhak, A. V., Pivovarenko, V. G., Klymchenko, A. S., Mely, Y. (2013) Dual-fluorescence L-amino acid reports insertion and orientation of melittin peptide in cell membranes. Bioconjug Chem, 24, 1998-2007.
2012
Strizhak, A. V., Postupalenko, V. Y., Shvadchak, V.V., Morellet, N. , Guittet, E. , Pivovarenko, V.G., Klymchenko , A.S., Mély, Y. (2012) Two-color fluorescent l-amino acid mimic of tryptophan for probing peptide–nucleic acid complexes. Bioconjug Chem. 23, 2434–43.

Giordano, L., Shvadchak, V. V., Fauerbach, J. A., Jares-Erijman, E. A., Jovin, T. M. (2012) Highly Solvatochromic 7-Aryl-3-hydroxychromones. J Phys Chem Lett 3, 1011-16 (52 cit.)

Pivovarenko, V. G., Zamotaiev, O. M., Shvadchak, V. V., Postupalenko, V. Y., Klymchenko, A. S. & Mely, Y. (2012). Quantification of local hydration at the surface of biomolecules using dual-fluorescence labels. J Phys Chem A 116, 3103-09.
2011
Shvadchak, V.V., Yushchenko, D.A., Pievo, R. and Jovin, T.M. (2011) The mode of alpha-synuclein binding to membranes depends on lipid composition and lipid to protein ratio. FEBS Lett. 585, 3513-9.
Shvadchak, V.V., Falomir-Lockhart, L.J., Yushchenko, D.A. and Jovin, T.M. (2011) Specificity and kinetics of alpha-synuclein binding to model membranes determined with fluorescent excited state intramolecular proton transfer (ESIPT) probe. J Biol Chem, 286, 13023-32.
Postupalenko, V. Y., Shvadchak, V. V., Duportail, G., Pivovarenko, V. G., Klymchenko, A. S., Mely, Y. (2011). Monitoring membrane binding and insertion of peptides by two-color fluorescent label. Biochim Biophys Acta 1808, 424-32.
Zamotaiev, O. M., Postupalenko, V. Y., Shvadchak, V. V., Pivovarenko, V. G., Klymchenko, A. S., Mely, Y. (2011). Improved Hydration-Sensitive Dual-Fluorescence Labels For Monitoring Peptide-Nucleic Acid Interactions. Bioconjug Chem. , 22, 101-07.
Greiner, V. J., Shvadchak, V., Fritz, J., Arntz, Y., Didier, P., Frisch, B., Boudier, C., Mely, Y., and de Rocquigny, H. (2011) Characterization of the mechanisms of HIV-1 Vpr(52-96) internalization in cells. Biochimie 93, 1647-58.
2010
Choulier, L., Shvadchak, V., Naidoo, A., Klymchenko, A., Mely, Y., Altschuh, D. (2010) A peptide-based fluorescent ratiometric sensor for quantitative detection of proteins. Anal. Biochem., 401, 188-95.
2009
Shvadchak, V.V., Klymchenko, A.S., de Rocquigny, H. and Mely, Y. (2009) Sensing peptide–oligonucleotide interactions by a two-color fluorescence label: application to the HIV-1 nucleocapsid protein. Nucleic Acids Res, e37, (3), e25.
Shvadchak, V.; Sanglier, S.; Rocle, S.; Villa, P.; Haiech, J.; Hibert, M.; Van Dorsselaer, A.; Mely, Y.; de Rocquigny, H. (2009) Identification by high throughput screening of small compounds inhibiting the nucleic acid destabilization activity of the HIV-1 nucleocapsid protein. Biochimie, 91, (7), 916-23.
Bilokin, M.D., Shvadchak, V.V., Yushchenko, D.A., Duportail, G., Mely, Y. and Pivovarenko, V.G. (2009) Dual-Fluorescence Probe of Environment Basicity (Hydrogen Bond Accepting Ability) Displaying no Sensitivity to Polarity. J Fluoresc., 19, (3), 545-53.
Bilokin, M.D., Shvadchak, V.V., Yushchenko, D.A., Klymchenko, A.S., Duportail, G., Mely, Y. and Pivovarenko, V.G. (2009) 3-Hydroxybenzo[g]quinolones: dyes with red-shifted absorption and highly resolved dual emission. Tetrah. Lett., 50, (30), 4714-9.
Spadafora, M., Postupalenko, V., Shvadchak, V.V., Klymchenko, Mely, Y., Burger, A., and Benhida, R. (2009) Efficient Synthesis of Ratiometric Fluorescent Nucleosides Featuring 3-Hydroxychromone Nucleobases Tetrahedron, 67, (37), 7809-16.
2008
Klymchenko, A.S., Shvadchak, V.V., Yushchenko, D.A., Jain, N. and Mely, Y. (2008) Excited-state intramolecular proton transfer distinguishes microenvironments in single- and double-stranded DNA. J Phys Chem B, 112, 12050-5.
de Rocquigny, H., Shvadchak, V., Avilov, S., Dong, C.Z., Dietrich, U., Darlix, J.L. and Mely, Y. (2008) Targeting the viral nucleocapsid protein in anti-HIV-1 therapy. Mini Rev Med Chem, 8, 24-35.
Avilov, S.V., Piemont, E., Shvadchak, V., de Rocquigny, H. and Mely, Y. (2008) Probing dynamics of HIV-1 nucleocapsid protein/target hexanucleotide complexes by 2-aminopurine. Nucleic Acids Res, 36, 885-96.
Pipko, S., Bezgubenko, L., Sinitsa, A., Rusanov, E., Kapustin, E., Povolotskii, M. and Shvadchak, V. (2008) Synthesis and structure of complexes of phosphorus pentachloride with 4-dimethylaminopyridine and N-methytimidazole. Heteroatom Chem, 19, 171-77.
2007
Yushchenko, D.A., Shvadchak, V.V., Klymchenko, A.S., Duportail, G., Pivovarenko, V.G. and Mely, Y. (2007) Modulation of excited-state intramolecular proton transfer by viscosity in protic media. J Phys Chem A, 111, 10435-38.
Yushchenko, D.A., Shvadchak, V.V., Klymchenko, A.S., Duportail, G., Pivovarenko, V.G. and Mely, Y. (2007) Steric control of the excited-state intramolecular proton transfer in 3-hydroxyquinolones: steady-state and time-resolved fluorescence study. J Phys Chem A, 111, 8986-92.
M’Baye, G., Klymchenko, A.S., Yushchenko, D.A., Shvadchak, V.V., Ozturk, T., Mely, Y. and Duportail, G. (2007) Fluorescent dyes undergoing intramolecular proton transfer with improved sensitivity to surface charge in lipid bilayers. Photochem Photobiol Sci, 6, 71-76.
2006
Yushchenko, D.A., Shvadchak, V.V., Bilokin, M.D., Klymchenko, A.S., Duportail, G., Mely, Y. and Pivovarenko, V.G. (2006) Modulation of dual fluorescence in a 3-hydroxyquinolone dye by perturbation of its intramolecular proton transfer with solvent polarity and basicity. Photochem Photobiol Sci, 5, 1038-44.
Yushchenko, D., Shvadchak, V., Klymchenko, A., Duportail, G., Mely, Y. and Pivovarenko, V. (2006) 2-Aryl-3-hydroxyquinolones, a new class of dyes with solvent dependent dual emission due to excited state intramolecular proton transfer. New J Chem, 30, 774-81.
Pipko, S., Simurova, N., Shvadchak, V., Bezgubenko, L. and Luk’yanenko, S. (2006) Dichlorophosphate anion in the synthesis of thioamides. Russ J Gen Chem+, 76, 1019-21.

Patent
Mely, Y., Langedijk, J.,  Shvadchak, V.,  Darlix, J-L. Peptides and antiviral drug, (2012) WO2012076822A1

 

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Vasyl Stefanyk Precarpathian National University