Dr. Nadiia Mosiichuk

Nadiia Mosiichuk, PhD, Associate Professor

I. ADDRESS

Work address: Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.

Phone: +38(0342)596171  

E-mail: sem_nad@ukr.net

II. PERSONAL

Ukrainian, female, born March 27, 1983 (Ukraine)

Languages: English, Ukrainian, Russian  

Family status: married

III. EDUCATION

2013: PhD degree in Biochemistry

2005-2008: Ph.D. student, Department of Biochemistry and Biotechnology. Ph.D. thesis “Antioxidant mechanisms in Arabidopsis thaliana lines impaired in tocopherol biosynthesis genes in response to stress factors”; 

2004-2005: Master’s degree in Chemistry, Department of Organic and Analytical Chemistry, Precarpathian National University. M.Sc. thesis “Effect of SiO2 supplementation on rheological and electrophysical properties of system”.

2004: Bachelor’s degree in Chemistry

2000-2004: Undergraduate student, Precarpathian National University. Obtained the diploma with honor.

IV. RESEARCH EXPERIENCE AND PRACTICAL TRAININGS

16/08/2015-23/09/2015: Visiting researcher, Kielanowski Institute of Animal Physiology and Nutrition, Jablonna, Poland. Research work related with investigation of dependence of exocrine pancreatic insufficiency from alpha ketoglutarate and chronic kidney diseases. 

07/06/2015-28/07/2015: Visiting researcher, Department of Biology, Faculty of Science, Lund University, Lund, Sweden. Research work devoted to studying of relation between diet and chronic kidney dysfunction development in pigs.

01/03/2013 – present:  Researcher, Laboratory of Biochemistry, Department of Biochemistry and Biotechnology, Precarpathian National University, Ukraine. Research work related with studying of pesticide impact on free radical processes, some biochemical and hematological parameters, and histological changes in different tissues of goldfish. 

15/09/2014-20/10/2014: Visiting researcher, Department of Biology, Faculty of Science, Lund University, Lund, Sweden. Research work related with studying of fat absorption in young pigs with exocrine pancreatic insufficiency.

01/09/2008-01/03/2013: Technician, Department of Biochemistry and Biotechnology, Precarpathian National University, Ukraine.

01/09/2005 – 01/09/2008: PhD student scientific work in Laboratory of Biochemistry, Department of Biochemistry, Precarpathian National University, Ukraine.

V. CURRENT AREAS OF INTEREST

– Oxidative stress and free radical processes in living organisms;

– Mechanisms of stress resistance in living organisms;

– Signaling in regulation of adaptation to stress;

– Effects of environmental pollutants and xenobiotics on living organisms;

VI. SKILLS

General

• Experience in article writing, analysis of literature;

• Experimental designing;

• Individual work or in the group;

• Teaching of younger colleagues;

• Tissue sampling from plant and fish.

Biochemistry

• Assay of enzyme activities (superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase, dehydroascorbate reductase, glutathion peroxidase, glutathione reductase, glutathione-S-transferase, ascorbate oxidase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase, acetylcholine esterase, alanine aminotransferase, aspartate aminotransferase);

• Determination of oxidative stress markers (thiobarbituric acid reactive substances, carbonyl proteins, lipid peroxides, thiols, glutathione);

• Measurement of chlorophylls, carotenoids and anthocyanins;

• Protein and DNA electrophoresis;

• Metabolomics (glucose, glycogen, TAG, lactate, uric acid, creatinine and protein).

Physiology

• Plants A. thaliana and Zea mays L. growing (on soil and hydroponics).  

• Fruit fly D. melanogaster and goldfish maintaining;

VII. PUBLICATIONS

Notice: the Last Name Semchuk has been changed with Moiichuk after the marriage.

Articles

1. Mosiichuk N.M., Husak V.V., Maksymiv I.V., Hlodan O.Y., Storey J.M., Storey K.B., Lushchak V.I. Toxicity of environmental Gesagard to goldfish may be connected with induction of low intensity oxidative stress in concentration- and tissue-related manners // Aquat Toxicol. – 2015. – Vol. 165. – P. 249-258. 

2. Maksymiv I.V., Husak V.V., Mosiichuk N.M., Matviishyn T.M., Sluchyk I.Y., Storey J.M., Storey K.B., Lushchak V.I. Hepatotoxicity of herbicide Sencor in goldfish may result from induction of mild oxidative stress // Pesticide Biochemistry and Physiology. – 2015. – Vol. 122. – P. 67-75.

3. Husak V.V., Mosiichuk N.M., Maksymiv I.V., Sluchyk I.Y., Storey J.M., Storey K.B., Lushchak V.I. Histopathological and biochemical changes in goldfish kidney due to exposure to the herbicide Sencor may be related to induction of oxidative stress // Aquatic Toxicology. –  2014. – Vol. 155. – P. 181-189.

4. Vasylyk  Y.V., Semchuk  N.M., Lushchak  Ok.V. Effect of sodium nitroprusside on activities of antioxidant and glutathione-related enzymes in leaves of maize seedlings // Scientific Herald of Chernivtsi University. Biology (Biological System). – Vol. 6, Is. 2. – Chernivtsi: Chernivtsi National University, 2014. –P. 167-172. 

5. Lushchak V.I., Semchuk N.M. Tocopherol biosynthesis: chemistry, regulation and effects of environmental factors // Acta Physiologiae Plantarum. – 2012. – Vol. 34(5). – P. 1607-1628.

6. Semchuk N.M., Vasylyk Y.V., Lushchak O.V., Lushchak V.I. Effect of short-term salt stress on oxidative stress markers and antioxidant enzymes activity in tocopherol-deficient Arabidopsis thaliana plants // Ukr Biokhim Zh. – 2012. –Vol. 84(4). – P. 41-48.

7. Vasylyk Y.V., Semchuk N.M., Lushchak O.V., Lushchak V.I. Effect of sodium chloride and nitroprusside on protein carbonyl groups content and antioxidant enzyme activity in leaves of corn seedlings Zea mays L // Ukr Biokhim Zh. – 2012. Vol. 84(3). – P. 82-87.

8. Semchuk N.M., Vasylyk Iu.V., Kubrak O.I., Lushchak V.I. Effect of sodium nitroprusside and S-nitrosoglutathione on pigment content and antioxidant system of tocopherol-deficient plants of Arabidopsis thaliana // Ukr Biokhim Zh. – 2011. – Vol. 83(6). – P. 69-79.

9. Semchuk N.M., Lushchak O.V., Falk J., Krupinska K., Lushchak V.I. Inactivation of genes, encoding tocopherol biosynthetic pathway enzymes, results in oxidative stress in outdoor grown Arabidopsis thaliana // Plant Physiology and Biochemistry. – 2009. – Vol. 47(5). – P. 384-390.

10. Semchuk N., Lushchak O.V., Falk J., Krupinska K., Lushchak V.I. Effect of VTE1 and VTE4 gene inactivation on salt stress response in Arabidopsis thaliana // Ukr Biokhim Zh. – 2008. Vol. 80(3). – P. 48-54.

Abstracts

1. Grujic D.,  Fedkiv O.,  Nikandrova  E.,  Mosiejchuk N.,  Kirko S.,  Osadchenko I.,   Kovalenko  T.,  Drahanchuk  O.,  Parshykov  O.,  Goncharova  E.,   Freedman S.,   Martin C.,   Pierzynowski  S. ePS05.1 Nutritional formula pre-hydrolyzed by novel point of care lipase device (EFIC™) leads to increased fat absorption in young pigs with exocrine pancreatic insufficiency (EPI). Abstracts of the 38th European Cystic Fibrosis Conference //Journal of Cystic Fibrosis. – 2015. – Vol. 14. – P. S50.

2. Maksymiv I. Oxidative stress development in goldfish kidney after exposure to metribuzin-containing herbicide “Sencor”/ I. Maksymiv, V. Husak, N. Mosiichuk // Abstracts book the X International Scientific Conference for Students and PhD [“Youth and Progress of Biology”] (Lviv, 8 – 11 April 2014). – Lviv, 2014. – P. 54-55.

3. Kindrat I. Tocopherol deficiency may influence oxidative stress response in Arabidopsis thaliana towards short-term salt stress / I. Kindrat, N. Semchuk // Materials of 5d International young scientists conference [Biodiversity. Ecology. Adaptation. Evolution.], (Odesa, 13-17 June 2011). – Odesa: Odesa National  I.I. Mechnykov University, 2011. – Р. 148.

4. Semanyk U. Pigment concentration in corn seedlings exposed to potassium dichromate/ U. Semanyk, N. Semchuk // Materials of 5d International young scientists conference [Biodiversity. Ecology. Adaptation. Evolution.], (Odesa, 13-17 June 2011). – Odesa: Odesa National  I.I. Mechnykov University, 2011. – Р. 170.

5. Semchuk N. Salt stress induces oxidative stress in Arabidopsis thaliana plants: possible involvement of tocopherols / N. Semchuk // Materials of 3rd International young scientists conference [Biodiversity. Ecology. Adaptation. Evolution.], (Odesa, 15-18 May 2007). – Odesa: Odesa National  I.I. Mechnykov University, 2007. – Р. 221.